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Merck

56156

Millipore

M-17-Bouillon

suitable for microbiology, NutriSelect® Basic, selective medium for Streptococcus thermophilus

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About This Item

UNSPSC-Code:
41171607
NACRES:
NA.85

Qualitätsniveau

Sterilität

non-sterile

Form

powder

Haltbarkeit

limited shelf life, expiry date on the label

Zusammensetzung

ascorbic acid, 0.5 g/L
lactose, 5 g/L
magnesium sulfate, 0.25 g/L
meat extract, 5 g/L
meat peptone (peptic), 2.5 g/L
sodium glycerophosphate, 19 g/L
soya peptone (papainic), 5 g/L
tryptone, 2.5 g/L
yeast extract, 2.5 g/L

Verpackung

pkg of 500 g

Hersteller/Markenname

NutriSelect® Basic

Methode(n)

microbiological culture: suitable

pH-Wert

7.0±0.2 (25 °C)

Anwendung(en)

food and beverages
microbiology

Lagertemp.

2-25°C

Eignung

nonselective for Enterococcus spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.

Verwandte Kategorien

Allgemeine Beschreibung

M-17 Broth is a selective medium for Streptococcus thermophilus in the common examination of yoghurt. It is superior to other comparable culture media for the cultivation of the fastidious species such as S. cremoris, S. diacetilactis and S. lactis. Mutants which are incapable of metabolizing lactose can also be isolated on these media. Tryptone, meat peptone, soya peptone, yeast extract and meat extract provide carbonaceous, nitrogenous compounds, vitamin B complex and other essential growth factors. Lactose is the fermentable carbohydrate and ascorbic acid is stimulatory for the growth of lactic Streptococci. Magnesium sulphate provides essential ions to the organisms. Sodium glycerophosphate increases the buffering capacity of the medium, this promotes the growth of lactic streptococci and inhibits lactobacilli growth.

Anwendung

Flüssiges Medium für die Selektion von Streptococcus thermophilus, welches in der Untersuchung von Yoghurt gebräuchlich ist. Wird auch in der milchverarbeitenden Industrie für die Anreicherung von Streptococcus thermophilus verwendet.

Angaben zur Herstellung

Suspend 42 g of powder in 1 litre of distilled water and let it soak. Heat to boiling and dispense into suitable containers. Sterilize by autoclaving at 121°C for 15 minutes.

Fußnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Simple and rapid method for isolating large plasmid DNA from lactic streptococci.Simple and rapid method for isolating large plasmid DNA from lactic streptococci.
Anderson, Douglas G., and Larry L. Mckay.
Applied and Environmental Microbiology, 46 (3), 549-552 (1983)

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