10110434001

Xanthine Oxidase (XOD)

from cow milk

• Synonym(e): XOD
• EC-Nummer: 1.1.3.22 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204

Eigenschaften

• Biologische Quelle: bovine milk
• Qualitätsniveau: 100
• Form: suspension
• Spezifische Aktivität: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• Verpackung: pkg of 1 mL (20 U)
• Hersteller/Markenname: Roche
• Optimaler pH-Wert: 8.5-9.0
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

Anwendung

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

Biochem./physiol. Wirkung

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Qualität

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Sequenz

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Einheitendefinition

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Physikalische Form

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Angaben zur Herstellung

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash