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MABS417

Sigma-Aldrich

Anti-GOT2, clone 3E9 Antibody

ascites fluid, clone 3E9, from mouse

Synonym(e):

Aspartate aminotransferase, mitochondrial, mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine-oxoglutarate transaminase 4, Kynurenine--oxoglutarate

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100 μL
324,00 €

324,00 €


Voraussichtliches Versanddatum23. Mai 2025


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100 μL
324,00 €

About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

324,00 €


Voraussichtliches Versanddatum23. Mai 2025


Bulk-Bestellung anfordern

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

3E9, monoclonal

Speziesreaktivität

mouse, rat, monkey, human

Methode(n)

immunofluorescence: suitable
western blot: suitable

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... GOT2(2806)

Allgemeine Beschreibung

Aspartate aminotransferase, mitochondrial ((mAspAT), also known as Fatty acid-binding protein (FABP-1) or Glutamate oxaloacetate transaminase 2 or Kynurenine aminotransferase 4 or Kynurenine aminotransferase IV or Kynurenine--oxoglutarate transaminase 4 or Kynurenine--oxoglutarate transaminase IV or Plasma membrane-associated fatty acid-binding protein (FABPpm) or Transaminase A, and encoded by the gene name GOT2, plays an essential role in role in amino acid metabolism and facilitates cellular uptake of long-chain free fatty acids. GOT2 is localized to the mitochondrion; however, exposure to alcohol promotes translocation to the cell membrane.

Immunogen

Purified recombinant fragment of human GOT2 expressed in E. Coli

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Stoffwechselhormone & Rezeptoren
Anti-GOT2, clone 3E9 Antibody is an antibody against GOT2 for use in western blotting & immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GOT2 in PC-3 and SK-BR-3 cells.

Qualität

Evaluated by Western Blotting in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GOT2 in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.

Zielbeschreibung

~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Jing Xu et al.
Translational stroke research, 11(3), 418-432 (2019-09-02)
The preservation of mitochondrial function is a major protective strategy for cerebral ischemic injuries. Previously, our laboratory demonstrated that protein kinase C epsilon (PKCε) promotes the synthesis of mitochondrial nicotinamide adenine dinucleotide (NAD+). NAD+ along with its reducing equivalent, NADH

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