MAB5350

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, mouse monoclonal, 28C4

• Synonym(e): ChAT
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Produktbezeichnung: Anti-Choline Acetyltransferase Antibody, clone 28C4, clone 28C4, Chemicon^®, from mouse
• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 28C4, monoclonal
• Speziesreaktivität: human, rat, guinea pig, monkey
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_020549.3; NM_020984.2; NM_020985.2; NM_020986.2
• UniProt-Hinterlegungsnummer: P28329
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CHAT(1103) ; rat ... Chat(290567) ; rhesus monkey ... Chat(709977)

Beschreibung

Spezifität

Reacts with Choline Acetyltransferase (ChAT).

Immunogen

Purified human placenta ChAT.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurotransmitter & Rezeptoren

Neuronen- & Gliamarker

Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.

Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.

Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.

ELISA (two side)

Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified

Purified immunoglobulin. Liquid.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable