MAB3408

Anti-Tubulin Antibody, beta, clone KMX-1

clone KMX-1, Chemicon^®, from mouse

• Synonym(e): Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: KMX-1, monoclonal
• Speziesreaktivität: human, rat, mouse
• Speziesreaktivität (Voraussage durch Homologie): all
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG2b
• NCBI-Hinterlegungsnummer: NM_006086.2
• UniProt-Hinterlegungsnummer: Q13509
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TUBB3(10381)

Beschreibung

Allgemeine Beschreibung

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.

Spezifität

This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.

Immunogen

Tubulin purified from polycephalum myxamoebae

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelett

Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

Western blot:
0.05-0.5 μg/mL

Optimal working dilutions must be determined by end user.

This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.

Qualität

Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.

Zielbeschreibung

50 kDa

Verlinkung

Replaces: 04-1049

Physikalische Form

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Hinweis zur Analyse

Control
HeLa Cell lysate

A431 Cell lysate

MCF7 Cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable