MAB1905

Anti-Laminin α1/β 1 Antibody, clone AL-4

clone AL-4, Chemicon^®, from rat

• Synonym(e): Laminin A Chain
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: AL-4, monoclonal
• Speziesreaktivität: mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_005559.2
• UniProt-Hinterlegungsnummer: P25391; P55268
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Lama1(16772)

Beschreibung

Spezifität

As original characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin α1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele et al (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kDa β1/γ1 band and the 400 kDa α1 band of mouse EHS laminin. However the affinity for the 400 kDa band was very low. AL-4 also demonstrated strong binding the Laminin β1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin β1 chain, however the clone also recognizes the laminin α1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins α1 and β1, with the β1 reactivity being more prominent.

Immunogen

Laminin isolated from mouse EHS tumor.

Anwendung

Research Category
Zellstruktur

Research Sub Category
ECM-Proteine

ELISA/RIA

Western blot

Immunoprecipitation

Immunohistochemistry. Reactive on tissue frozen in OCT at -70°C. Sections may be treated with hyaluronidase. Shows reactivity to paraffin embedded EHS tumor.

Optimal working dilutions must be determined by the end user.

This Anti-Laminin α1/β 1 Antibody, clone AL-4 is validated for use in ELISA, IP, WB, IH(P) for the detection of Laminin α1/β 1.

Physikalische Form

Format: Purified

Purified from ascites by Protein G affinity chromatography. Purified immunoglobulin in 0.02M PB, 0.25M NaCl, pH=7.6, 0.1% sodium azide

Lagerung und Haltbarkeit

Maintain between 2 and 8°C.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable