IPFL10100

Immobilon^® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

• Synonym(e): Western blotting membrane, blotting membrane, transfer membrane
• UNSPSC Code: 41105339
• eCl@ss: 32031602
• NACRES: NB.22
• Material: PVDF membrane, plain filter, white filter
• Detection method: chemiluminescent, colorimetric, fluorometric
• Pore size: 0.45 μm pore size
• Compatibility: for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

Eigenschaften

• Produktname: Immobilon^®-FL PVDF Membrane, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
• Quality Level: 400
• material: PVDF membrane, plain filter, white filter
• feature: hydrophobic
• manufacturer/tradename: Immobilon^®
• technique(s): dot blot: suitable, western blot: suitable
• filter L × W: 10 cm × 10 cm
• pore size: 0.45 μm pore size
• capacity: 155 μg/cm² adsorption capacity (insulin), 205 μg/cm² adsorption capacity (BSA), 300 μg/cm² adsorption capacity (goat IgG)
• compatibility: for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
• detection method: chemiluminescent, colorimetric, fluorometric
• shipped in: ambient

Beschreibung

General description

The Immobilon^®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon^®-FL membrane has a nominal pore size of 0.45 μm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.

Application

Immobilon^®-FL membrane has been used in western blotting.[1][2][3] It is also suitable for use in standard chemiluminescent or chromogenic detection.

Features and Benefits

Immobilon^®-FL Membrane:

• The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
• Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
• compatible with standard blocking agents and buffers
• Ideal for multiplexing and chemifluorescence detections

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable