AB1922
Anti-Integrin β4 Antibody
serum, Chemicon®
Synonym(e):
CD104
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100 μL
390,00 €
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100 μL
390,00 €
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Speziesreaktivität
human, mouse, rat
Hersteller/Markenname
Chemicon®
Methode(n)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ITGB4(3691)
mouse ... Itgb4(192897)
rat ... Itgb4(25724)
Spezifität
Specific for beta4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits.
Immunogen
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human β4 integrin subunit (Tamura et al. 1990). The sequence used is NH2-(K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the β4 sequence and was added to conjugate the peptid
Anwendung
Anti-Integrin β4 Antibody is an antibody against Integrin β4 for use in IP, IH.
Immunoprecipitation from labeled cell extracts: 5 μL of serum is sufficient to precipitate alpha6beta4 from 1-2 x 10E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells results in two bands of 95 and 55 kDa of unknown origin in addition to the 200 kDa and 140 kDa bands corresponding to beta4 and alpha6.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Haiyao Wang et al.
The Journal of biological chemistry, 289(44), 30318-30332 (2014-09-10)
Epithelial cells are highly dependent during wound healing and tumorigenesis on the α6β4 integrin and its association with receptor tyrosine kinases. Previous work showed that phosphorylation of the β4 subunit upon matrix engagement depends on the matrix receptor syndecan (Sdc)-1
Palmitoylation by DHHC3 is critical for the function, expression, and stability of integrin ?6?4.
Sharma, C; Rabinovitz, I; Hemler, ME
Cellular and Molecular Life Sciences null
Haiyao Wang et al.
The Journal of biological chemistry, 290(43), 26103-26113 (2015-09-10)
The α6β4 integrin is known to associate with receptor tyrosine kinases when engaged in epithelial wound healing and in carcinoma invasion and survival. Prior work has shown that HER2 associates with α6β4 integrin and syndecan-1 (Sdc1), in which Sdc1 engages
Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
Chen, J; Jing, L; Gilchrist, CL; Richardson, WJ; Fitch, RD; Setton, LA
Connective tissue research null
K Triantafilou et al.
Journal of virology, 74(13), 5856-5862 (2000-06-14)
Human parechovirus 1 (HPEV1) displays an arginine-glycine-aspartic acid (RGD) motif in the VP1 capsid protein, suggesting integrins as candidate receptors for HPEV1. A panel of monoclonal antibodies (MAbs) specific for integrins alphavbeta3, alphavbeta1, and alphavbeta5, which have the ability to
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