5.04744

Aphidicolin

≥98% (TLC), liquid, DNA polymerase α and δ inhibitor, Calbiochem

• Synonym(e): InSolution Aphidicolin
• Empirische Formel (Hill-System): C₂₀H₃₄O₄
• CAS-Nummer: 38966-21-1
• Molekulargewicht: 338.48
• MDL-Nummer: MFCD00083214
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• product name: Aphidicolin, InSolution, ≥98%, 30 mM in DMSO, A cell-permeable tetracyclic diterpene antibiotic
• Qualitätsniveau: 100
• Assay: ≥98% (TLC)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Lagertemp.: −70°C
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• InChIKey: NOFOAYPPHIUXJR-APNQCZIXSA-N

Beschreibung

Allgemeine Beschreibung

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific, non-competitive and reversible inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine (>Cat. No. 677181)-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
DNA polymerase α and δ

Reversible: yes

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin (Cat. No. 178273) in DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions.

Sonstige Hinweise

Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene10, 185.
Urbani, L., et al. 1995. Exp. Cell Res.219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol.46, 1909.Hubermann, J.A. 1981. Cell23, 647.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F - (refers to pure substance)
• Flammpunkt (°C): 87 °C - (refers to pure substance)