495624

Olomoucine

InSolution, ≥98%, potent and selective inhibitor of p34cdc2/cyclin B and related kinases

• Synonym(e): InSolution Olomoucine
• Empirische Formel (Hill-System): C₁₅H₁₈N₆O
• Molekulargewicht: 298.34
• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥98% (HPLC)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; desiccated (hygroscopic); protect from light
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

A purine derivative that acts as a potent and selective inhibitor of p34cdc2/cyclin B (IC₅₀ = 7 µM) and related kinases, including p33cdk2/cyclin A (IC₅₀ = 7 µM), p33cdk2/cyclin E (IC₅₀ = 7 µM), p33cdk5/p35 (IC₅₀ = 3 µM), and p44MAPK (IC₅₀ = 25 µM). Acts by competing for the ATP binding domain of the kinase. Exhibits reduced sensitivity towards related kinases, p34cdk4/cyclin D1 (IC₅₀ >1 mM) and p40cdk6/cyclin D3 (IC₅₀ >250 µM). Does not significantly affect the activity of other protein kinases even at 1 mM concentrations. Known to inhibit DNA synthesis in interleukin-2 stimulated T lymphocytes and triggers G₁ arrest similar to that observed in interleukin-2 deprived cells. Also used to synchronize cells in G₁.

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
p34^cdk1/cyclin B

Product competes with ATP.

Reversible: yes

Target IC₅₀: 100 nM-100 µM against p34cdk1/cyclin B

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

A 50 mM (5 mg/336 µl) solution of Olomoucine (Cat. No. 495620) in DMSO.

Rekonstituierung

Following initial use, aliquot and refrigerate (4°C).

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flammpunkt (°C): 87 °C - closed cup - (Dimethylsulfoxide)