235427

Caspase-3 Inhibitor I, Cell-Permeable

InSolution, ≥90%

• Synonym(e): InSolution Caspase-3 Inhibitor I, Cell-Permeable, Cell-permeable, DEVD-CHO; CPP32/Apopain Inhibitor, Cell-permeable
• Empirische Formel (Hill-System): C₉₄H₁₅₈N₂₀O₂₇
• Molekulargewicht: 2000.38
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥90% (HPLC)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; desiccated (hygroscopic); protect from light
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (K_i<1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC₅₀ = 200 pM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
caspase-3

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 200 pM inhibiting PARP cleavage in cultured human osteosarcoma cell extracts

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Irritant (B)

Sequenz

Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO

Physikalische Form

A 5 mM (1 mg/100 µl) solution of Caspase-3 Inhibitor I, Cell-permeable (Cat. No. 235423) in DMSO.

Rekonstituierung

Following initial thaw, aliquot, purge with inert gas, and freeze (-20°C).

Sonstige Hinweise

Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem.271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem.270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature371, 346.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flammpunkt (°C): 87 °C - closed cup - (Dimethylsulfoxide)