1.19643

Superspher^® 60 RP-select B (4 µm) HPLC Columns

HPLC sorbent

• Synonym(e): TM=["LiChrospher"] C8 sorbent for HPLC
• UNSPSC-Code: 41115709
• NACRES: SB.52

Eigenschaften

• product name: Superspher^® 60 RP-Select B,
• Materialien: stainless steel column
• Agentur: suitable for USP L7
• Produktlinie: Superspher^®
• Leistungsmerkmale: endcapped: no
• Parameter: 60 °C max. temp.
• Methode(n): HPLC: suitable; mass spectrometry (MS): suitable (Low - Medium)
• Matrix: fully porous particle
• Aktive Matrixgruppe: C8 bonding phase
• Partikelgröße: 4 μm
• Porengröße: 60 Å pore size
• operating pH range: 2-7.5
• Schüttdichte: 200‑400 kg/m³
• Trenntechnik: reversed phase
• Lagertemp.: no temp limit

Beschreibung

Allgemeine Beschreibung

Superspher^® RP-select B ist ein leicht reproduzierbarer, traditionell hergestellter, sphärischer Kieselgelträger mit Umkehrphaseneigenschaften und mit der Hydrophobie einer C-8-Phase. Der Träger eignet sich sehr gut für die Trennung von basischen Verbindungen, weist jedoch auch gute Eigenschaften für die Bestimmung neutraler und saurer Verbindungen auf. Dieser Hochleistungs-Kieselgelträger mit einer Partikelgröße von 4 µm bietet ein hervorragendes Verhältnis von Druck und Separationsleistung.

Anwendung

• Screening, library-assisted identification and validated quantification of 23 benzodiazepines, flumazenil, zaleplone, zolpidem and zopiclone in plasma by liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization.: This study discusses a comprehensive method for the detection and quantification of various benzodiazepines in plasma using liquid chromatography/mass spectrometry, highlighting the utility of Superspher^® 60 RP-select B columns in achieving high sensitivity and specificity (Kratzsch et al., 2004).



• Determination of heterocyclic aromatic amines in beef extract, cooked meat and rat urine by liquid chromatography with coulometric electrode array detection.: This research demonstrates the application of Superspher^® 60 RP-select B in the precise measurement of heterocyclic aromatic amines in biological samples, emphasizing its role in enhancing detection sensitivity and accuracy (Gerbl et al., 2004).



• Screening, library-assisted identification and validated quantification of fifteen neuroleptics and three of their metabolites in plasma by liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization.: This paper presents a validated method for detecting neuroleptics in plasma, utilizing Superspher^® 60 RP-select B columns to achieve efficient separation and accurate quantification (Kratzsch et al., 2003).



• Validated assay for quantification of oxcarbazepine and its active dihydro metabolite 10-hydroxycarbazepine in plasma by atmospheric pressure chemical ionization liquid chromatography/mass spectrometry.: This study outlines a method for quantifying oxcarbazepine and its metabolite in plasma, using Superspher^® 60 RP-select B columns to ensure high reproducibility and reliability in the assay (Maurer et al., 2002).



• Determination of the N-methyl-D-aspartate receptor NR2B subunit blocker Ro 63-1908 in rat, cynomolgus monkey and human plasma by high-performance liquid chromatography with column switching and fluorescence detection.: This research showcases the application of Superspher^® 60 RP-select B in the sensitive and specific determination of NMDA receptor blockers in various biological matrices, enhancing analytical performance (Wyss et al., 2000).

Hinweis zur Analyse

Anwendungstechnischer Test: entspricht
Druck: ≤ 70 bar
Theoretische Bodenzahl (N/m) (1,2-Benzo[a]anthracen): ≥ 110.000
Kapazitätsfaktor (1,2-Benzo[a]anthracen): 3,5–5,0
Stabilitätstest (Leistung): entspricht
MPPH/Diphenhydramin*HCL: 2,25–2,75
MPPH/Acetylsalicylsäure: 3,06–3,74
Toluol/MPPH: 2,37–2,63
Kapazitätsfaktor (Toluol): 11,05–14,95

Rechtliche Hinweise

Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable