d0-DMDSSO (dimethyl-disuccinimidyl sulfoxide) crosslinker is a homobifunctional, lysine residue-targeting, sulfoxide-containing crosslinker for analysis of protein-protein interactions (PPIs) through crosslinking mass spectrometry (XL-MS). d0-DMDSSO possesses two N-hydroxysuccinimide (NHS) ester groups for targeting amines and two symmetrical C-S cleavable bonds adjacent to the central sulfoxide. The post-cleavage spacer yields tagged peptides for unambiguous identification by collision-induced dissociation in tandem MS. d0-DMDSSO crosslinker will also be a useful proteomics tool and aid in the quest for targeting ″undruggable″ protein targets.
Structural characterization of protein complexes is essential for the understanding of their function and regulation. However, it remains challenging due to limitations in existing tools. With recent technological improvements, cross-linking mass spectrometry (XL-MS) has become a powerful strategy to define
Sulfoxide-containing MS-cleavable cross-linkers capture protein-protein interactions in native cellular environments, aiding PPI identification.
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