YEAST1
Yeast Transformation Kit
reagents for introducing plasmid DNA into yeast
Synonym(s):
lithium acetate yeast transformation
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Please contact Customer Service for Availability | 13 100,00 CZK |
About This Item
NACRES:
NA.85
UNSPSC Code:
12352200
Grade:
Molecular Biology
Technique(s):
molecular cloning: suitable, transformation: suitable
13 100,00 CZK
Please contact Customer Service for Availability
grade
Molecular Biology
Quality Segment
usage
kit sufficient for >100 standard transformations
technique(s)
molecular cloning: suitable, transformation: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.
Application
Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.
Biochem/physiol Actions
Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).
Features and Benefits
- Easy and ready-to-use
- Requires as little as 10 ng of plasmid DNA
- Flexibility for any strain of yeast
- Sufficient for over 100 standard transformations
Other Notes
The Yeast Transformation Kit contains:
- Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
- Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
- Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
- Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
- Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
1 of 1
This Item | |||
|---|---|---|---|
| description reagents for introducing plasmid DNA into yeast | description - | description without histidine, leucine, tryptophan, and adenine | description without lysine |
| technique(s) transformation: suitable | technique(s) - | technique(s) transformation: suitable | technique(s) - |
| grade Molecular Biology | grade - | grade - | grade - |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. room temp |
| usage kit sufficient for >100 standard transformations | usage - | usage - | usage - |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
WGK
WGK 3
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