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W4125

Sigma-Aldrich

Williams′ Medium E

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Medium E, Williams E Medium, Williams’ E medium

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10 X 1 L
CZK 1,750.00

CZK 1,750.00

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10 X 1 L
CZK 1,750.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

CZK 1,750.00

Please contact Customer Service for Availability
Request a Bulk Order

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.

Application

Williams′ E medium has been used in culturing mice/rat primary hepatocytes.[1][2][3]

Quantity

Formulated to contain 10.8 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000356042
0000355899
0000356042
0000272790
SLCP1438

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Etienne Guillocheau et al.
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Trans-palmitoleic acid (trans-C16:1 n-7 or trans-Δ9-C16:1, TPA) is believed to improve several metabolic parameters according to epidemiological data. TPA may mainly come from direct intakes: however, data are inconsistent due to its very low amount in foods. Instead, TPA might
M Herranz-Itúrbide et al.
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Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions
Xue Yu et al.
Acta biochimica et biophysica Sinica, 41(12), 1027-1032 (2009-12-17)
Homocysteine is an intermediate in the sulfur amino acid metabolism. Recent studies suggested that there might be links between hyperhomocysteinemia and insulin resistance. In the present study, we investigated the effect of homocysteine on glucose metabolism. We demonstrated that the
D M Toivola et al.
Journal of cell science, 110 ( Pt 1), 23-33 (1997-01-01)
The importance of protein phosphatases in the maintenance of cytoskeletal structure is supported by the serious liver injury caused by microcystin-LR, a hepatotoxic inhibitor of type-1 and type-2A serine/threonine protein phosphatases. We used the microcystin-LR-induced cell injury as a model
Yingjiang Zhou et al.
The Journal of biological chemistry, 284(17), 11152-11159 (2009-03-05)
Major urinary protein (MUP) 1 is a lipocalin family member abundantly secreted into the circulation by the liver. MUP1 binds to lipophilic pheromones and is excreted in urine. Urinary MUP1/pheromone complexes mediate chemical communication in rodents. However, it is unclear
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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