Skip to Content
Merck

Key Documents

View All Documentation

Skip To

N6635

Nutrient Mixture F-10 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Ham’s F-10

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
10 x 1 L
Please contact Customer Service for Availability
CZK 1,740.00
10 L
Please contact Customer Service for Availability
CZK 1,770.00
CZK 1,504.50

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217607

CZK 1,740.00

Please contact Customer Service for Availability
​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

General description

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Application

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit[1]
  • to culture satellite cells from fish white muscle of trout[2]
  • for culturing neonatal porcine islets (NPIs)[3]

Preparation Note

Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
N6013N2147N6908
Sigma-Aldrich

Sigma-Aldrich

N6635

Nutrient Mixture F-10 Ham

Nutrient Mixture F-10 Ham With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N6013

Nutrient Mixture F-10 Ham

Nutrient Mixture F-10 Ham With 20 mM HEPES, without sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N2147

Nutrient Mixture F-10 Ham

Nutrient Mixture F-10 Ham With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

N6908

Nutrient Mixture F-10 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

liquid

form

liquid

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

HEPES: 20 mM
L-glutamine: no
sodium pyruvate: 0.11 g/L
phenol red: yes
NaHCO3: no

components

L-glutamine: yes
HEPES: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Low serum media

Human diploid cell culture, low serum

Human diploid cell culture, low serum

Low serum media

Still not finding the right product?

Explore all of our products under Nutrient Mixture F-10 Ham

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000502092
0000502092
0000500474
0000500474
0000500476

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

Nutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

Amirhosein Hasani et al.
Life sciences, 254, 117767-117767 (2020-05-15)
Heat stress shock affects the generation of free radicals and can have a harmful effect on spermatogenesis. Photobiomodulation (PBM) is very effective in andrology for treating male infertility. This research aimed at the evaluation of the impacts of PBM on
M A Silvestre et al.
Theriogenology, 58(1), 69-76 (2002-08-17)
Vitrification (3.58 M EG and 2.82 M DMSO in PBS with 20% FCS) and rapid-freezing (0.25 M sucrose, 2.25 M EG, and 2.25 M DMSO in PBS with 20% FCS) procedures were assayed to cryopreserve rabbit tissue samples from 12-day
Ensieh Sajadi et al.
Journal of cellular biochemistry, 120(10), 17312-17325 (2019-05-22)
The current study was conducted to assess the relationship between testicular cells in spermatogenesis, through which the production of healthy and mature sperm is essential. However, it seems necessary to obtain more information about the three-dimensional pattern of the testis
View All Related Papers

Global Trade Item Number

SKUGTIN
N6635-10X1L04061835569045
N6635-10L04061834117636

Questions

  1. While preparing media solution from nutrient mixture F-10 Ham powder, is it recommended to add antifungal and antibiotic in media solution? If so, what amount of it should be added in 1L of media?

    1 answer

    1. This product does not contain antibiotics or antifungals by default. The addition of these constituents is at the discretion of the end user. The final concentration is dependent upon the type of product used – penicillin, streptomycin, etc. Several examples are listed below:
      P0781, Penicillin-Streptomycin, 10,000 units penicillin and 10 mg streptomycin per mL, add 10 mL per liter medium
      https://www.sigmaaldrich.com/product/sigma/p0781

      P4458, Penicillin-Streptomycin, 5,000 units penicillin and 5 mg streptomycin per mL, add 20 mL per liter medium
      https://www.sigmaaldrich.com/product/sigma/p4458

      A5955, Penicillin-Streptomycin-Amphotericin B Suspension, Stabilized, with 10,000 units penicillin, 10 mg streptomycin and 25 ?g amphotericin B per mL, add 10 mL per liter medium
      https://www.sigmaaldrich.com/US/en/product/sigma/a5955

      N1638, Nystatin Suspension, suspension, 10,000 unit/mL, add 10 mL per liter medium
      https://www.sigmaaldrich.com/product/sigma/n1638

      Helpful?

Reviews

No rating value

Active Filters