V5890

Vascular Endothelial Growth Factor 121 human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• UNSPSC Code: 12352202
• NACRES: NA.26
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: monomer 18 kDa (glycosylated)
• Impurities: ≤1 EU/μg Endotoxin level

Properties

• Product Name: VEGF121, recombinant, expressed in HEK 293 cells, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• description: Vascular Endothelial Growth Factor 121 human
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: <10 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: monomer 18 kDa (glycosylated)
• packaging: pkg of 10 μg, pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg Endotoxin level
• color: white
• solubility: water: soluble
• UniProt accession no.: P15692
• storage temp.: −20°C
• Gene Information: human ... VEGFA(7422)

Description

General description

VEGF121 is expressed in human HEK 293 cells as a disulfide linked, glycosylated cytokine with an apparent molecular mass of 37 kDa as a homodimer and 50 kDa as a homotrimer. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Biochem/physiol Actions

VEGF121 belongs to the PDGF/VEGF growth factor family characterized by the presence of eight conserved cysteine residues and a cystine knot structure.[1] VEGF is secreted by the majority of tumor cells and initiates angiogenesis by activating endothelial cells of existing blood vessels and promoting their migration.[2]
VEGF has also been implicated in correlation with poor prognosis in breast cancer.[2]
In addition, VEGF is released in rheumatoid arthritis in response to TNF-α, increasing endothelial permeability and stimulating angiogenesis (formation of capillaries).[3][4]

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable