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U0251

Urease from Canavalia ensiformis (Jack bean)

Type C-3, powder, ≥600,000 units/g solid

Synonym(s):

Jack bean urease, Urea amidohydrolase

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Size/SKUAvailabilityPrice
6000 units
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CZK 4,860.00
12000 units
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CZK 6,630.00
60000 units
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CZK 24,000.00

About This Item

CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
≥600,000 units/g solid
Biological source:
Canavalia ensiformis

CZK 4,860.00


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biological source

Canavalia ensiformis

Quality Segment

type

Type C-3

form

powder

specific activity

≥600,000 units/g solid

mol wt

~544620 Da

purified by

crystallization

storage temp.

−20°C

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a nickel-dependent metalloenzyme , found in various plants, bacteria, fungi, and algae. It contains two distinct subunits-UreA(26.5 kDa) and UreB (60.3–61.6 kDa).

Biochem/physiol Actions

Urease aids in the bioavailability of nitrogen in plants. It is implicated in defense mechanism and exhibits insecticidal activity. In addition, urease exhibits antifungal activity by retarding growth and affecting membrane integrity of filamentous fungi and yeasts.
Urease was shown to induce vacuolation in Hela cells, an effect dependent on the ability of urease to convert urea to ammonia. [1]

Other Notes

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

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This Item
94280U1875U7752
specific activity

≥600,000 units/g solid

specific activity

~1 U/mg

specific activity

-

specific activity

-

biological source

Canavalia ensiformis

biological source

-

biological source

Canavalia ensiformis

biological source

Canavalia ensiformis

form

powder

form

powder

form

glycerol solution

form

solid

mol wt

~544620 Da

mol wt

Mr ~480000

mol wt

~544620

mol wt

hexamer 545 kDa, trimer ~272 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

purified by

crystallization

purified by

-

purified by

-

purified by

-


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1



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