This material, T9265, is traceable to the WHO 3rd International Standard 81/565 and to WHO 80/558, with a typical activity of 7 IU/mg.
T9265
Thyrotropic hormone from human pituitary
lyophilized powder, ≥6.2 IU/mg (immunoassay, non-sterile)
Synonym(s):
hTSH, TSH, Thyroid stimulating hormone
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| Size/SKU | Availability | Price |
|---|---|---|
5 μg | Please contact Customer Service for Availability | CZK 4,440.00 |
10 μg | Please contact Customer Service for Availability | CZK 7,740.00 |
25 μg | Please contact Customer Service for Availability | CZK 16,100.00 |
0.1 mg | Please contact Customer Service for Availability | CZK 51,400.00 |
About This Item
biological source
human pituitary glands
Quality Segment
sterility
non-sterile
form
lyophilized powder
specific activity
≥6.2 IU/mg (immunoassay, non-sterile)
storage condition
(Keep container tightly closed in a dry and well-ventilated place)
technique(s)
cell culture | mammalian: suitable
impurities
HIV Antibodies
Antibody to HCV
Antibody to HBV or Presence of HBSAG, tested negative
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... TSHB(7252), TSHR(7253)
General description
Research area: Cell Signaling
Thyrotropic hormone or thyroid stimulating hormone (TSH) belongs to the glycoprotein hormone family. Thyrotroph cells of the anterior pituitary gland produces TSH and is stored in the secretory granules. It comprises a heterodimer of two noncovalently linked subunits, α and β.
Thyrotropic hormone or thyroid stimulating hormone (TSH) belongs to the glycoprotein hormone family. Thyrotroph cells of the anterior pituitary gland produces TSH and is stored in the secretory granules. It comprises a heterodimer of two noncovalently linked subunits, α and β.
Application
Thyrotropic hormone from human pituitary has been used as a supplement in the fetal thyroid organoid culture medium for stimulating maturation.[1] It has also been used as a medium supplement for primary cell culture, and is used further to study the molecular mechanisms of estrogen-mediated growth stimulation of human thyroid tumor cells.[2]
Biochem/physiol Actions
Two-chain glycoprotein hormone. The α-chain is not active, biological specificity attributed rather to the β-chain. Activates adenylate cyclase in the thyroid gland, stimulating iodine uptake, thyroxine synthesis and release. Goitrogenic.
The product can enhance vascular endothelial growth factor (VEGF) secretion and angiogenesis in thyroid cancer celllines.[3]TSHR activated by TSH activates both the adenosine 3′,5′-cyclic monophosphate (cAMP) pathway (via Gsa) and the phosphoinositol/calcium (IP/Ca2+; via Gq) second messenger signaling pathway by interacting with Gs and Gq G-proteins. In mature adipocytes, TSH aids in lipid metabolism by inhibiting fatty acid synthase (FASN) expression via, activating protein kinase A (PKA)-dependent phosphorylation of extracellular signal-regulated kinases/p42/p44 (ERK1/2), and c-Jun N-terminal kinase (JNK).[4]
In children, thyroid hormones are very much essential for the normal cognition and neurodevelopment. Thyroid-stimulating hormone (TSH) test is mainly used to diagnose thyroid function.
Packaging
Package size based on hormone
Physical form
Contains hormone and salts from approx. 0.1 mL of 0.05 M phosphate buffer, pH 7.4.
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This Item | |||
|---|---|---|---|
| biological source human pituitary glands | biological source bovine pituitary glands | biological source bovine pituitary glands | biological source human |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) activity assay: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form powder | form powder | form powder |
| Gene Information human ... TSHB(7252), TSHR(7253) | Gene Information cow ... TSHB(281552) | Gene Information - | Gene Information human ... TRH(7200), TRHR(7201) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is this material traceable to WHO 3rd IS 81/565?
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How to reconstitute this product.
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This product may be reconstituted in 0.01M phosphate buffer at pH 7.5. Stock solutions, prepared at 10 micro IU per milliliter, may be stored frozen in aliquots. Avoid repeated freeze/thaw cycles. Working solutions should be prepared fresh and not stored.
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