T6778

Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Goat Anti-Rabbit IgG (whole molecule)–Tetramethylrhodamine isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162790
• Conjugate: TRITC conjugate
• Clone: polyclonal
• Application: IF (d), IHC (p)
• Citations: 103

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: TRITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• contains: ≥15 mM sodium azide as preservative
• storage condition: protect from light
• technique(s): direct immunofluorescence: 1:400, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Binds all rabbit Igs.

IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds.

Application

Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry.

Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques.[1]

Biochem/physiol Actions

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Preparation Note

Adsorbed to reduce background staining with human samples.

Other Notes

Antibody adsorbed with human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)