T6319

Anti-TOM22 antibody , Mouse monoclonal

clone 1C9-2, purified from hybridoma cell culture

• Synonym(s): Anti-Mitochondrial import receptor Tom22, Anti-TOMM22, Anti-Translocase of outer mitochondrial membrane 22 homolog (yeast)
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03457578
• Conjugate: unconjugated
• Clone: 1C9-2, monoclonal
• Application: ARR, ICC, WB
• Citations: 13

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 1C9-2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 22 kDa
• species reactivity: human, monkey
• should not react with: rodent
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: suitable, microarray: suitable, western blot: 0.5-1.0 μg/mL using whole extract of cultured human lymphoma Raji cells.
• isotype: IgG1
• UniProt accession no.: Q9NS69
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TOMM22(56993)

Description

General description

Translocase of outer mitochondrial membrane 22 (TOMM22) is an essential receptor of the mitochondrial import channel. It is characterized with an N-terminal negatively charged region exposed to the cytosol, a putative transmembrane region and a C-terminal intermembrane space region with little negative charge.

Immunogen

membrane fraction from Vero (monkey kidney-derived) cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

• Western blotting

Biochem/physiol Actions

Translocase of outer mitochondrial membrane 22 (TOMM22) is a key constituent of TOM complex. It functions as a mitochondrial receptor for the proapoptotic protein Bax (BCL2-associated X protein). TOMM22 interacts with 3-β hydroxysteroid dehydrogenase 2 (3βHSD2) and converts pregnenolone-to-progesterone in steroidogenic cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable