T1270

Thiamine hydrochloride

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

• Synonym(s): Aneurine hydrochloride, Vitamin B₁ hydrochloride
• Empirical Formula (Hill Notation): C₁₂H₁₇ClN₄OS · HCl
• CAS Number: 67-03-8
• Molecular Weight: 337.27
• NACRES: NA.75
• PubChem Substance ID: 24899952
• eCl@ss: 34058006
• UNSPSC Code: 12352207
• EC Number: 200-641-8
• MDL number: MFCD00012780
• Beilstein/REAXYS Number: 3851771

Properties

• biological source: synthetic (organic)
• Quality Segment: 200
• product line: BioReagent
• assay: ≥99.0% (HPLC)
• form: powder
• mol wt: M_w 337.27 g/mol
• technique(s): cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
• color: white
• mp: 250 °C (dec.) (lit.)
• solubility: H₂O: 50 mg/mL, clear, colorless
• application(s): agriculture
• SMILES string: CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]
• InChI: 1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1
• InChI key: DPJRMOMPQZCRJU-UHFFFAOYSA-M

Description

General description

Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain.[1] Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge.

Application

Thiamine hydrochloride has been used as a component:

• of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii[2]
• of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis[3]
• of modified Bold 3N medium for culturing Chlorella minutissima[4]

Biochem/physiol Actions

Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase.[1] Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway.[5] Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome.[6] Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)