S9564

Amyloid Precursor Protein α, Secreted human

>90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), buffered aqueous solution

• Synonym(s): sAPPα
• MDL number: MFCD03454663
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli (N-terminal histidine tagged)
• Quality Level: 200
• Assay: >90% (SDS-PAGE)
• form: buffered aqueous solution
• mol wt: ~100 kDa by SDS-PAGE
• UniProt accession no.: P05067
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... APP(351)

Description

General description

The APP (amyloid precursor protein) gene is mapped to human chromosome 21q21.3.[1] It encodes a integral membrane protein. APPα is a soluble protein generated by sequential cleavage with α and γ secretase.[2]

Application

Human amyloid precursor protein α secreted, has been used in enzyme linked immunosorbent assay (ELISA).[3][4][5]

Biochem/physiol Actions

Amyloid precursor protein α is an α-secretase-cleaved soluble protein that has been shown to have neuroprotective properties. It is derived from amyloid precursor protein. The protein consists of 612 amino acids. Several G protein-coupled receptors are known to activate α-secretase-dependent processing of APP. It has neuroprotective, neurogenic and neurotrophic functions. Amyloid precursor protein a also stimulates gene expression and protein expression.[6]

Physical form

Solution 0.2 μm filtered, in phosphate buffered saline, pH 7.4.

Preparation Note

Expressed as a soluble protein and purified under non-denaturing conditions.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable