The fractionated proteins are suitable for downstream proteomic applications. Unfortunately, activity and native conformation of proteins have not been evaluated, and would not be guaranteed.
PE0240
Plant Fractionated Protein Extraction Kit
Suitable for any plant species or tissue
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Please contact Customer Service for Availability | CZK 14,000.00 |
About This Item
NACRES:
NA.56
UNSPSC Code:
41105500
Usage:
sufficient for 20 extractions
Storage temp.:
−20°C
General description
Sigma′s Plant Fractionated Protein Extraction Kit is designed specifically for use in plant bioscience to extract qualitative hydrophilic and hydrophobic proteins samples from any type of plant species or tissue for downstream proteomic applications. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes five reagents; a plant specific protease inhibitor, a specially formulated reagent to extract hydrophilic proteins and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins. Also included in the kit are reducing reagent Tributylphosphine (TBP) and alkylating reagent, Iodoacetamide. These reagents improve separation during isoelectric focusing and 2-D gel electrophoresis. The protease inhibitor is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases. The cocktail contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, pepstatinA, E-64, leupeptin, and 1,10-phenanthroline.
Following removal of polyphenolics, tannins and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of hydrophilic proteins, addition of the chaotropic reagent provided extracts hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and protein extract solutions are collected. The end results are qualitative fractionated protein samples, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of hydrophilic proteins, addition of the chaotropic reagent provided extracts hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and protein extract solutions are collected. The end results are qualitative fractionated protein samples, ready for downstream proteomic analysis.
1 of 1
This Item | |||
|---|---|---|---|
| usage sufficient for 20 extractions | usage - | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
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Related Content
Instructions
Loomis, W.D.
Methods in Enzymology, 31, 528-545 null
Yinghui Ying et al.
Plant physiology, 173(1), 812-824 (2016-11-30)
Phosphate overaccumulator2 (PHO2) encodes a ubiquitin-conjugating E2 enzyme that is a major negative regulator of the inorganic phosphate (Pi)-starvation response-signaling pathway. A yeast two-hybrid (Y2H) screen in rice (Oryza sativa; Os) using OsPHO2 as bait revealed an interaction between OsPHO2
M Ferro et al.
Electrophoresis, 21(16), 3517-3526 (2000-11-18)
As a complementary approach to genome projects, proteomic analyses have been set up to identify new gene products. One of the major challenges in proteomics concerns membrane proteins, especially the minor ones. A procedure based on the differential extraction of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PE0240-1KT | 04061825657448 |
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Hi, can the protein extracted by this kit (Plant Fractionated Protein Extraction Kit: PE0240) be used for pull-down or ip? Does the extracted protein remain active？
1 answer-
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