P9208

Polymyxin Acylase from Pseudomonas sp.

≥0.1 units/mg solid

• Synonym(s): Peptide N-fatty acylase
• CAS Number: 111174-43-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00286307
• Specific activity: ≥0.1 units/mg solid
• Biological source: bacterial (Pseudomonas spp.)

Properties

• biological source: bacterial (Pseudomonas spp.)
• Quality Segment: 200
• form: solid
• specific activity: ≥0.1 units/mg solid
• relevant disease(s): cancer
• storage temp.: −20°C

Description

Application

Polymyxin acylase, from Pseudomonas sp., is a N-myristoyl cleaving enzyme that has been used to determine the N-myristoyl peptide sequence [1] and may be useful in cancer research since it has antitumor activity against murine and human tumor cells [2].

Biochem/physiol Actions

Polymyxin acylase, from Pseudomonas sp. deacylates polymyxin group antibiotics and long-chain fatty acyl groups of proteins. Polymyxin acylase has an affinity for long-chain fatty acyl proteins in human carcinoma cells [2].

Analysis Note

Crude actone powder;

Other Notes

One unit will hydrolyze 1 μmole of N-octanoyl-5-aminovaleric acid per min at pH 8.0 at 37°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves