P7749

Anti-Profilin 1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-PFN1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD09265369
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, WB CL
• Citations: 14

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~15 kDa
• species reactivity: human, rat, mouse
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 10-20 μg/mL using rat NRK cells, western blot (chemiluminescent): 1-2 μg/mL using whole extracts of mouse NIH3T3 and human HeLa cells
• UniProt accession no.: P15735
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PFN1(5216) ; mouse ... Pfn1(18643) ; rat ... Pfn1(64303)

Description

General description

Anti-Profilin 1 (N-terminal) is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acid residues of human profilin 1, conjugated to keyhole limpet hemocyanin (KLH). Profilin 1 is a ubiquitous actin monomer-binding protein. Profilin 1 is highly expressed throughout development and adulthood in most of the tissues including brain.

Immunogen

synthetic peptide corresponding to amino acid residues 2-17 of human profilin 1, conjugated to KLH. The correspopnding rat and mouse sequence differs by one amino acid. This sequence is 70% similar to the corresponding sequence in profilin 2.

Application

Anti-Profilin 1 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Profilin 1 is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility.. Profilin 1 is a potent regulator of actin filament dynamics. Profilin 1 was suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when overexpressed in breast cancer cells. Deletion of profilin 1 gene leads to an embryonic lethal phenotype and Miller-Dieker syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)