P2980

Pertussis toxin

from Bordetella pertussis, buffered aqueous glycerol solution, protein endotoxin

• Synonym(s): Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen
• CAS Number: 70323-44-3
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00131841
• Form: buffered aqueous glycerol solution

Properties

• Product Name: Pertussis toxin from Bordetella pertussis, buffered aqueous glycerol solution
• description: aseptically packaged
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC
• InChI: 1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3
• InChI key: WOPKHAQDUMDJIY-UHFFFAOYSA-N
• Gene Information: Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)

Description

General description

Pertussis (Pertussis) toxin (PTX) is a protein isolated from the bacterium Bordatella (Bordatella) coccobaccillus. The toxin is excreted in a non-active state. When the B oligomer of the bacterium, Bordatella coccobacillus, comes in contact with the cell membrane, an S1 subunit of its A protomer is activated. This activation may be mediated by glutathione and/or ATP.

Application

Inactive toxin form released from B. pertussis.

Pertussis toxin from Bordetella pertussis has been used: to enhance cardiac myosin immunization of rats to induce myocarditis[1]; to break down the blood–brain barrier in the Cuprizone and cuprizone/MOG-EAE model;[2] as an inhibitor of Gαi signaling[3]

The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins G_i, G_o, and G_t.

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins G_i, G_o, and G_t. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the G_α subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K⁺ channels.

Features and Benefits

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Physical form

Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable