P1743

Protein Phosphatase 2C from bovine brain

buffered aqueous glycerol solution

• CAS Number: 362690-38-8
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD04041076
• Specific activity: ~1000 units/mg protein
• Assay: >90% (SDS-GE)
• Biological source: bovine brain

Properties

• biological source: bovine brain
• Quality Segment: 200
• assay: >90% (SDS-GE)
• form: buffered aqueous glycerol solution
• specific activity: ~1000 units/mg protein
• mol wt: 42-45 kDa
• packaging: vial of 1 μg
• technique(s): ligand binding assay: suitable
• UniProt accession no.: P67774
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: bovine ... PPP2CA(282320)

Description

General description

Protein phosphatase 2C (PP2C) is a member of protein phosphatases, Mg²⁺/Mn²⁺ dependent (PPM) family. PP2C is a monomeric enzymes found in both prokaryotes and eukaryotes.

Biochem/physiol Actions

In Arabidopsis, protein phosphatase 2C (PP2C) negatively regulates plant hormone, abscisic acid (ABA) signal transduction. In eukaryotes, this protein modulates the cell cycle by reversing the activating phosphorylation of cyclin-dependent protein kinases (CDKs). PP2C acts as a crucial physiological regulators of cell growth and of cellular stress signaling.

Physical form

Solution in 50 mM Tris-HCl, pH 7.0, containing 14 mM 2-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 50% glycerol

Other Notes

One unit will release 1.0 nanomole of phosphate from ³²P-labeled myelin basic protein at pH 7.0 at 30 °C

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable