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Phosvitin from egg yolk

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About This Item

CAS Number:

9008-96-2

UNSPSC Code:

12352202

NACRES:

NA.81

MDL number:

MFCD00131908

Form:

powder

Biological source:

egg yolk

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Properties

biological source

egg yolk

Quality Segment

200

form

powder

storage temp.

−20°C

Description

General description

Phosvitin from egg yolk is a polyanionic phosphoserine rich protein.[1] This phosphoglycoprotein comprises α-helices and β-sheets structural elements, which are poorly organized due to the presence of phosphoserine.[1] It represents about 11% of yolk proteins and is hydrophilic.[2]

Application

Phosvitin from egg yolk has been used:

as a control protein for immobilization on-chip for deep purple protein staining methods for monitoring post-translation modifications[3]
as a substrate for casein kinase I_ε in in vitro casein kinase assay[4]
as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantification of phosvitin from egg extracts[5]
for immobilization onto Layer-by-Layer (LbL) for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies[6]

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus. [7]

Phosvitin from egg yolk is a calcium chelator[1] and binds to iron present in the yolk.[2] The metal-binding functionality is exploited in mineral-binding bioactive peptide production.[2] It is a nutraceutical and has potential for phosphopeptides production.[2]

Other Notes

A phosophoprotein containing 8-10% phosphorus. Molar N/P ratio approx. 2.7.

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This Item	A9207	C7786	L4754
Sigma-Aldrich P1253 Phosvitin from egg yolk	Sigma-Aldrich A9207 Avidin−Agarose from egg white	Sigma-Aldrich C7786 Conalbumin from chicken egg white	Sigma-Aldrich L4754 3-sn-Lysophosphatidylethanolamine from egg yolk
biological source egg yolk	biological source egg white	biological source chicken egg white	biological source egg yolk
form powder	form aqueous glycerol suspension	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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