To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Synonym(s):
Alkaline phosphatase-Agarose
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| Size/SKU | Availability | Price |
|---|---|---|
50 units | Please contact Customer Service for Availability | 6 920,00 CZK |
100 units | Please contact Customer Service for Availability | 11 900,00 CZK |
250 units | Please contact Customer Service for Availability | 25 400,00 CZK |
1000 units | Please contact Customer Service for Availability | 79 900,00 CZK |
About This Item
Specific activity:
250-1000 units/mL packed gel
Biological source:
bovine (calf) intestine
6 920,00 CZK
Please contact Customer Service for Availability
biological source
bovine (calf) intestine
Quality Segment
form
ammonium sulfate suspension
specific activity
250-1000 units/mL packed gel
matrix
beaded agarose
storage temp.
2-8°C
General description
Application
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine.
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases.
Biochem/physiol Actions
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Packaging
Package size based on DEA units
Physical form
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Other Notes
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
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This Item | |||
|---|---|---|---|
| description ammonium sulfate suspension | description suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml) | description buffered aqueous solution, ≥2,000 DEA units/mg protein | description BioUltra, ≥5,700 DEA units/mg protein |
| biological source bovine (calf) intestine | biological source bovine (calf) intestinal mucosa | biological source bovine intestinal mucosa | biological source - |
| specific activity 250-1000 units/mL packed gel | specific activity ~2500 U/mg protein (~10 mg/ml) | specific activity ≥2,000 DEA units/mg protein | specific activity ≥5,700 DEA units/mg protein |
| form ammonium sulfate suspension | form solution (clear, colorless) | form buffered aqueous solution | form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| matrix beaded agarose | matrix - | matrix - | matrix - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
7783-20-2
CAS No.
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1. What is the recommended speed for spinning down the phosphatase, alkaline-agarose when using this product?
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