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79390

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Synonym(s):

Alkaline Phosphatase from calf intestinal mucosa

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Size/SKUAvailabilityPrice
1 mg
Please contact Customer Service for Availability
7 380,00 CZK
5 mg
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27 000,00 CZK

About This Item

CAS Number:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
MFCD00131849
EC Number:
3.1.3.1 (BRENDA, IUBMB)
Specific activity:
~2500 U/mg protein (~10 mg/ml)
Biological source:
bovine (calf) intestinal mucosa

7 380,00 CZK

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biological source

bovine (calf) intestinal mucosa

form

solution (clear, colorless)

specific activity

~2500 U/mg protein (~10 mg/ml)

technique(s)

enzyme immunoassay: suitable, protein detection: suitable

impurities

0.1 mM ZnCl2, 1 mM MgCl2, 3 M NaCl, 30 mM triethanolamine

pH

~7.6

storage temp.

2-8°C

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Preparation Note

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
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Phosphatase, Alkaline from calf intestinal mucosa suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Sigma-Aldrich

79390

Phosphatase, Alkaline from calf intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, ≥2,000 DEA units/mg protein

Sigma-Aldrich

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Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, ≥5,700 DEA units/mg protein

Sigma-Aldrich

P0114

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline−Agarose from calf intestine ammonium sulfate suspension

Sigma-Aldrich

P0762

Phosphatase, Alkaline−Agarose from calf intestine

description

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

description

buffered aqueous solution, ≥2,000 DEA units/mg protein

description

BioUltra, ≥5,700 DEA units/mg protein

description

ammonium sulfate suspension

technique(s)

enzyme immunoassay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

specific activity

~2500 U/mg protein (~10 mg/ml)

specific activity

≥2,000 DEA units/mg protein

specific activity

≥5,700 DEA units/mg protein

specific activity

250-1000 units/mL packed gel

biological source

bovine (calf) intestinal mucosa

biological source

bovine intestinal mucosa

biological source

-

biological source

bovine (calf) intestine

form

solution (clear, colorless)

form

buffered aqueous solution

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

pH

~7.6

pH

-

pH

-

pH

-

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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