N8523

Monoclonal Anti-NF-κB antibody produced in mouse

clone NF-12, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00802846
• Conjugate: unconjugated
• Clone: NF-12, monoclonal
• Application: ARR, ICC, WB
• Citations: 13

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: NF-12, monoclonal
• mol wt: antigen 65 kDa
• contains: 15 mM sodium azide
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable, microarray: suitable, western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line
• isotype: IgG1
• UniProt accession no.: P19838
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NFKB1(4790) ; mouse ... Nfkb1(18033)

Description

General description

Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors.

Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.

Immunogen

recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids).

Application

Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting.

Biochem/physiol Actions

Nuclear factor kappa-B (NF-κB1) has an ability to affect transcription of κB reporter genes positively or negatively when assayed following transfection.

Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable