The volume may be determined using the lot specific Enzymatic Activity reported in the Certificate of Analysis. For example, if a specific lot reports an Enzymatic Activity of 7.17 U/mL, the 1 unit pack size will have a volume of roughly 139 uL and the 2 unit pack size would have a volume of 278 uL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/n7885#product-documentation
N7885
Neuraminidase from Vibrio cholerae
Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
Synonym(s):
Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
1 unit | Please contact Customer Service for Availability | CZK 4,520.00 |
2 units | Please contact Customer Service for Availability | CZK 8,480.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
Specific activity:
1-5 units/mg protein (Lowry, using NAN-lactose)
Quality Segment
type
Type III
form
buffered aqueous solution
specific activity
1-5 units/mg protein (Lowry, using NAN-lactose)
mol wt
83 kDa
application(s)
diagnostic assay manufacturing
foreign activity
Protease and NAN-aldolase, present
storage temp.
2-8°C
General description
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
Application
Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. [1] It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase. [2]
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.
Biochem/physiol Actions
Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. [1]
Physical form
Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.
Preparation Note
Chromatographically purified.
Analysis Note
Preservative free.
Other Notes
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 1-5 units/mg protein (Lowry, using NAN-lactose) | specific activity ≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA) | specific activity ≥50 units/mg protein (using 4MU-NANA) | specific activity ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin) |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| form buffered aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt 83 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| foreign activity Protease and NAN-aldolase, present | foreign activity Protease and NAN-aldolase, present | foreign activity - | foreign activity Protease and NAN-aldolase, present |
Still not finding the right product?
Explore all of our products under Neuraminidase from Vibrio cholerae
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Dear Customer service I have a question regarding the product (N7885 Neuraminidase from Vibrio cholerae). In how much volume does the 1 Unit or 2 Unit package arrive? Could you provide a volume (or mg if it's powder)? Thanks, Ben
1 answer-
Helpful?
-