N5631

Neuraminidase from Clostridium perfringens (C. welchii)

Type VIII, lyophilized powder, ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)

• Synonym(s): Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• CAS Number: 9001-67-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-624-6
• MDL number: MFCD00131711
• EC Number: 3.2.1.18 (BRENDA, IUBMB)
• Specific activity: ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)

Properties

• type: Type VIII
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)
• foreign activity: Protease and NAN-aldolase, present
• storage temp.: −20°C
• Gene Information: Clostridium perfringens str. 13 ... nanI(988807)

Description

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Clostridium perfringens has been used in a study to assess the binding characteristics of iota toxin by fluorescence-activated cytometry. [1] It has also been used in a study to investigate the distribution of neuraminidase among food poisoning strains.

Biochem/physiol Actions

Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.

Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.

The degradation of gangliosides grown in lipid mono layers by Clostridium perfringens neuraminidase depends largely on surface pressure. Increased pressure can inhibit neuraminidase activity. [2]

The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

Preparation Note

Chromatographically purified from Type V (N 2876)

Analysis Note

Package sizes based on 4MU-NANA units

Package sizes based on the 4MU-NANA units

Other Notes

One unit will liberate 1.0 micromole of N-acetyl neuraminic acid per minute at pH 5.0 at 37 °C using bovine submaxillary mucin.

One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl)-a-D-N-actetylneuraminic acid per minute at pH 5.0 at 37 °C (using 4MU-NANA as a substrate)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)