N5389

Anti-Neurofilament H (200 kDa) Antibody

mouse monoclonal, NE14

• Synonym(s): NF200 Antibody - Monoclonal Anti-Neurofilament 200 antibody produced in mouse, Neurofilament Antibody, Nf200 Antibody, Monoclonal Anti-Neurofilament heavy chain
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02263444
• Conjugate: unconjugated
• Clone: NE14, monoclonal
• Application: ARR, IHC (f), IHC (p), WB
• Citations: 84

Properties

• Product Name: Monoclonal Anti-Neurofilament 200 antibody produced in mouse, clone NE14, ascites fluid
• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: NE14, monoclonal
• mol wt: antigen apparent mol wt 200 kDa
• contains: 15 mM sodium azide as preservative
• species reactivity: pig, mouse, chicken, bovine, guinea pig, rabbit, rat, human
• enhanced validation: independent ( Antibodies); Learn more about Antibody Enhanced Validation
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, immunohistochemistry (frozen sections): suitable, microarray: suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: P12036
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEFH(4744)

Description

General description

Monoclonal Anti-Neurofilament 200 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The neurofilaments are one of the five major groups of intermediate filaments (IFs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD and are named as NEFL (light), NEFM (medium) and NEFH (heavy) respectively. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals.

Immunogen

pig spinal cord.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-Neurofilament 200 antibody produced in mouse has been used in

• immunohistochemistry
• immunolabeling
• immunofluorescence

Biochem/physiol Actions

Neurofilament heavy chain (NEFH) gene was recently identified to cause autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc). This gene plays a role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable