N218

Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse

clone NOS-3F7-B11-B5, ascites fluid

• Synonym(s): Anti-IHPS1, Anti-N-NOS, Anti-NC-NOS, Anti-NOS, Anti-bNOS, Anti-nNOS
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01324876
• Conjugate: unconjugated
• Clone: NOS-3F7-B11-B5, monoclonal
• Application: IHC (f), WB
• Citations: 9

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: NOS-3F7-B11-B5, monoclonal
• species reactivity: mouse, bovine, rat
• technique(s): immunohistochemistry (frozen sections): 1:100, western blot: 1:50-1:500
• isotype: IgM
• UniProt accession no.: P29476
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Nos1(18125) ; rat ... Nos1(24598)

Description

Immunogen

bovine cerebellum NOS.

Biochem/physiol Actions

Neuronal nitric acid synthase (Nos1) in involved in the production of nitric oxide. Mutations in the gene encoding it increases the susceptibility to behavioral dysfunction after an exposure to alcohol in mice.

The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).

Physical form

Ascites fluid containing ≤0.1% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)