This would be an acceptable solvent as the DMSO concentration does not exceed 1%.. Please ensure the inhibitor is soluble in these conditions and perform the assay as instructed.
MAK458
Hyaluronidase Inhibitor Screening Assay Kit
Sufficient for 100 turbidimetric tests
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Please contact Customer Service for Availability | 14 600,00 CZK 11 972,00 CZK |
About This Item
NACRES:
NA.77
UNSPSC Code:
12161503
11 972,00 CZK
List Price14 600,00 CZKSave 18%Please contact Customer Service for Availability
Quality Segment
application(s)
advanced drug delivery
pharmaceutical
detection method
turbidimetric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Hyaluronidases are a family of enzymes that catalyze the degradation of the glycosaminoglycan, hyaluronic acid. Hyaluronic acid is one of the major constituents of the extracellular matrix in organisms where it contributes to both cell proliferation and migration. The role of hyaluronidases in breaking down this key factor in cell proliferation makes them a possible target for cancer treatment. One hypothesis is that increased hyaluronidase may help prevent tumor invasion by breaking down the extracellular matrix needed for tumor expansion. Conversely, decreasing hyaluronidase activity might prevent metastasis by stopping cancer cells from escaping primary tumor masses. The study to determine hyaluronidases′ exact role in cancer pathology is still ongoing.
Application
The Hyaluronidase Inhibitor Screening Assay Kit may be used for:
- Cancer Research
- Drug Discovery
The kit is suitable for the high-throughput screening for evaluation of hyaluronidase inhibitors.
Biochem/physiol Actions
The Hyaluronidase Inhibitor Screening Assay Kit uses a two step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at A600 nm is directly proportional to the hyaluronidase activity in the sample.
Features and Benefits
Efficient Inhibitor Screening: Screen for Hyaluronidase inhibitors using only 20 μL of test compound with a quick 30 minute protocol, offering a streamlined and effortless procedure.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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This Item | |||
|---|---|---|---|
| description Sufficient for 100 turbidimetric tests | description sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests | description Sufficient for 100 colorimetric tests |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 100 |
| detection method turbidimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| application(s) advanced drug delivery | application(s) advanced drug delivery | application(s) - | application(s) cosmetics |
| relevant disease(s) cancer | relevant disease(s) neurological disorders, Alzheimer′s disease | relevant disease(s) cancer | relevant disease(s) cardiovascular diseases; infectious diseases; neurological disorders |
Storage Class
12 - Non Combustible Liquids
WGK
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Manual says "Dissolve the test compounds (i.e., inhibitors) in solvent of choice." is it possible to use Ultrapure water + 1% DMSO to dissolve the inhibitors samples?
1 answer-
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What compounds are used as controls for the assay?
1 answer-
This assay includes a no inhibitor control (NIC) well containing 40 µL of hyaluronidase and 20 µL of the solvent used with the test compounds. Additionally, there is a no enzyme control (NEC) well containing 40 µL of enzyme buffer and 20 µL of the solvent used with the test compounds.
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