MAK161

Multidrug Resistance Assay Kit

(Fluorometric MDR Assay)

• Synonym(s): MDR Assay
• NACRES: NA.84
• UNSPSC Code: 12161503

Properties

• usage: (Fluorometric MDR Assay)
• application(s): pharmaceutical
• detection method: fluorometric
• relevant disease(s): cancer
• storage temp.: −20°C
• Gene Information: human ... ABCB1(5243), ABCC2(1244) ; mouse ... ABCC2(12780) ; rat ... abcc2(25303)

Description

General description

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.[1]

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_ex = 490/λ_em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Application

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

Biochem/physiol Actions

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_Ex = 490/λ_Em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Features and Benefits

Compatible with high-throughput handling systems.

Safety Information

• Storage Class: 10 - Combustible liquids