M8822

MEK1, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MAP2K1, MAPKK1, MKK1, PRKMK1
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 276-374 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 276-374 nmol/min·mg
• mol wt: ~69 kDa
• UniProt accession no.: P31938
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... Map2k1(26395)

Description

General description

Mitogen activated protein kinase/ERK kinase (MEK)1 is a hydrophilic non-receptor protein and is a member of dual specificity protein kinase family. It is ubiquitously expressed.[1] MAP2K1 is encoded by the gene mapped to human chromosome 15q22.31.[2]

Application

MEK1, active, GST tagged human has been used to induce kinase reactions in in vitro mitogen activated protein kinase/ERK kinase (MEK)1 kinase assay.[3] It has also been used to activate extracellular-signal-regulated kinase 2 (ERK2) by phosphorylation.[4]

Biochem/physiol Actions

Mitogen activated protein kinase/ERK kinase (MEK)1/2 catalyzes the phosphorylation of tyrosine and then threonine in extracellular-signal-regulated kinase1/2. As an essential component of RAS−RAF−MEK−ERK signaling pathway (MAP kinase signaling cascade), it is involved in the regulation of apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation.[1] Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative diseases such as cancer.[5]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)