These beads demonstrate an approximate Excitation/Emission of 481/644 nm, please see the link below to review the product specifications:
https://www.sigmaaldrich.com/specification-sheets/764/990/L9904-BULK.pdf
L9904
Latex beads, amine-modified polystyrene, fluorescent orange
aqueous suspension, 0.1 μm mean particle size
Synonym(s):
Amine-Modified Latex Beads, Fluorescent Orange Beads
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mL | Please contact Customer Service for Availability | CZK 2,120.00 |
About This Item
CZK 2,120.00
Please contact Customer Service for Availability
form
aqueous suspension
Quality Segment
composition
Solids, 2.5%
technique(s)
cell based assay: suitable
mean particle size
0.1 μm
fluorescence
λex ~475 nm; λem ~540 nm
application(s)
cell analysis
Application
Latex beads, amine-modified polystyrene, fluorescent orange has been used in the preparation of nano-particles in biophysical characterization. It has also been used in the preparation of nano-particles for cytotoxicity studies in yeast and cell lines.
Amine-modified polystyrene latex beads have been used to develop an electrochemical nitrite nanosensor as well as to validate a pharyngeal aspiration technique for exposing the mouse lung to respirable particles.
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This Item | |||
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) - | technique(s) cell based assay: suitable | technique(s) - |
| fluorescence λex ~475 nm; λem ~540 nm | fluorescence λex ~520 nm; λem ~540 nm | fluorescence λex ~520 nm; λem ~540 nm | fluorescence λex ~520 nm; λem ~540 nm |
| application(s) cell analysis | application(s) - | application(s) cell analysis | application(s) - |
| form aqueous suspension | form aqueous suspension | form aqueous suspension | form aqueous suspension |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
| composition Solids, 2.5% | composition Solids, 2.4-2.6% | composition Solids, 2.4-2.6% | composition Solids, 2.5% |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Edwin H Shin et al.
Nanoscale, 5(13), 5879-5886 (2013-05-24)
The use of nanoparticles for cellular therapeutic or sensing applications requires nanoparticles to bind, or adhere, to the cell surface. While nanoparticle parameters such as size, shape, charge, and composition are important factors in cellular binding, the cell itself must
Esen Efeoglu et al.
The Analyst, 142(18), 3500-3513 (2017-08-24)
Nanotoxicology has become an established area of science due to growing concerns over the production and potential use of nanomaterials in a wide-range of areas from pharmaceutics to nanomedicine. Although different cytotoxicity assays have been developed and are widely used
Exposure of the yeast Saccharomyces cerevisiae to functionalized polystyrene latex nanoparticles: influence of surface charge on toxicity
Nomura T, et al.
Environmental Science & Technology, 47(7), 3417-3423 (2013)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L9904-1ML | 04061838106162 |
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What is the correct emission wavelength? The product page on the website (https://www.sigmaaldrich.com/NL/en/product/sigma/l9904) says 540 nm, but the specification sheet mentions 644 nm (https://www.sigmaaldrich.com/specification-sheets/764/990/L9904-BU.
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is it possible to know the molecoar weight to calculate the molarity of the suspension?
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Unfortunately, the molecular weight of this product is not determined.
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How did you verify the amine coating? What spectroscopy method have you used to verify the amine coating? Can you show the result that proves that the amine has been coated? (For example, if you have done FTIR, then can you show us the peaks)
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The amine modification of these beads is validated through an extensive series of titrations. Unfortunately, the method is considered proprietary. There is no specific documentation or data available to share.
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what is the concentration of polystyrene microplastic [mg/ml] in this product No. L9904
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The concentration of this aqueous suspension is 2.5% w/v or 2.5g/100 mL. This equates to 25mg per 1 mL package.
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