Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae) and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.
Application
Suitable for inducing phagocytosis and screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells.
Zymosan A fluorescent particles were prepared by conjugating Zymosan A particles with a bright red fluorescent molecular probe. The particles are ready-to-use phagocytosis-inducing particles for fluorescent imaging, microplate reader (Ex/Em = 540/570 nm) and flow cytometric analysis of phagocytosis. We recommend using 5 μL of Zymosan A fluorescent particles per 5 x 105 of viable cells.
Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
description
This Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human Embryonic stem & induced pluripotent Stem cells.
storage temp.
2-8°C
storage temp.
room temp
storage temp.
2-8°C
storage temp.
-
suitability
suitable for fluorescence
suitability
-
suitability
-
suitability
-
concentration
1 mg/mL
concentration
-
concentration
-
concentration
-
Quality Level
100
Quality Level
200
Quality Level
100
Quality Level
100
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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