343209

Fluorescein Diacetate

≥99% (HPLC), solid, esterase substrate, Calbiochem^®

• Synonym(s): Fluorescein Diacetate
• Empirical Formula (Hill Notation): C₂₄H₁₆O₇
• CAS Number: 596-09-8
• Molecular Weight: 416.38
• UNSPSC Code: 12171500
• NACRES: NA.77
• MDL number: MFCD00005062
• Form: solid
• Storage condition: OK to freeze, protect from light

Properties

• Product Name: Fluorescein Diacetate, Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
• Quality Segment: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: colorless to yellow
• solubility: ethanol: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: O1C3(c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C)c2c(cccc2)C1=O
• InChI: 1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
• InChI key: CHADEQDQBURGHL-UHFFFAOYSA-N

Description

General description

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.

Substrate for lipases and esterases. Abs. Max: 498 nm.

Preparation Note

Unstable in solution. Reconstitute just prior to use.

Other Notes

Clements, K.M., et al. 2001. Osteoarthritis Cartilage9, 499.
Bedner, E., et al. 1998. Cytometry33, 1.
Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable