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I9278

Sigma-Aldrich

Insulin solution human

sterile-filtered, BioXtra, suitable for cell culture

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5 ML
CZK 5,900.00
100 ML
CZK 68,700.00

CZK 5,900.00

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5 ML
CZK 5,900.00
100 ML
CZK 68,700.00

About This Item

CAS Number:
11061-68-0
MDL number:
MFCD00131380
UNSPSC Code:
12352209
NACRES:
NA.77
form:
solution

CZK 5,900.00

Please contact Customer Service for Availability
Request a Bulk Order

sterility

sterile-filtered

Quality Level

400

product line

BioXtra

form

solution

concentration

9.5-11.5 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mL endotoxin

pH

7.9-8.5

UniProt accession no.

P01308

storage temp.

2-8°C

InChI key

PBGKTOXHQIOBKM-FHFVDXKLSA-N

Gene Information

human ... INS(3630)

General description

Insulin is a two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond.

Application

Insulin solution human has been used as a supplement in Dulbecco′s modified eagle′s medium (DMEM) to culture epithelial cell line and human endometrial cell line, minimum essential medium eagle to culture human breast adenocarcinoma cell line and medium 199 to culture normal ovarian cell line.
Insulin solution human is recommended for use in cell culture applications at 0.5 to 1mL per liter of medium.
Insulin solution human has been used for insulin tolerance test in mice and rats.[1][2] It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).[3][4]

Biochem/physiol Actions

Insulin plays a role in various cellular processes, such as promoting cell growth, regulating the cell cycle, and facilitating glucose uptake. It is commonly used as a protein supplement in serum-free mammalian cell culture media to help promote the optimal growth and maintenance of mammalian cells.

Other Notes

InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1

Physical form

stable 2-8 °C

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000341617
0000327021
0000327021
0000341617
0000258141

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Farinaz Ghodrati et al.
Scientific reports, 8(1), 8654-8654 (2018-06-07)
At times, it can be difficult to discern if a lack of overlap in reported interactions for a protein-of-interest reflects differences in methodology or biology. In such instances, systematic analyses of protein-protein networks across diverse paradigms can provide valuable insights.
E Comisso et al.
Oncogene, 36(30), 4253-4266 (2017-03-21)
OCT4 (Octamer-binding transcription factor 4) is essential for embryonic stem cell self-renewal. Here we show that OCT4 increases the aggressiveness of high-grade serous ovarian cancer (HG-SOC) by inactivating the Retinoblastoma tumor suppressor pathway and enhancing mitotic stability in cancer cells.
Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.
Jones M et al.
Cytotherapy, 15, 1323-1323 (2013)
Early cardiac changes in a rat model of prediabetes: brain natriuretic peptide overexpression seems to be the best marker.
Nunes S, et al.
Cardiovascular Diabetology, 12, 44-44 (2013)
Hui-Yu Liu et al.
The Journal of biological chemistry, 284(40), 27090-27100 (2009-08-06)
Subjects with type 1 diabetes mellitus (T1DM) eventually develop insulin resistance and other features of T2DM such as cardiovascular disorders. The exact mechanism has been not been completely understood. In this study, we tested the hypothesis that excessive or inappropriate
View All Related Papers

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Questions

1–10 of 15 Questions  

  1. How is this insulin produced? In E.Coli? Yeast?...?

    1 answer

    1. This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.

      Helpful?

  2. Is this product suitable for insulin tolerance tests in mice

    1 answer

    1. This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/

      Helpful?

  3. How long does the insulin remain potent for if stored in the fridge?

    1 answer

    1. This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/i9278#product-documentation

      Helpful?

  4. Is this insulin considered of fast or slow action?

    1 answer

    1. This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
      Insulin Glargine - Slow acting Insulin
      https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=product

      Aspart Insulin - Fast acting Insulin
      https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=product

      Lispro Insulin - Fast acting Insulin
      https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=product

      Helpful?

  5. Is this human insulin suitable for use in mouse cell culture?

    1 answer

    1. Yes, this product is suitable for mouse cell culture and has been cell culture tested on the L929 and 3T3 mouse cell lines. Please see the link below to review peer-reviewed articles that may be of interest:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892261/#:~:text=This%20process%20requires%20DRAM%2D1,and%20permitting%20efficient%20mTORC1%20activation.

      Helpful?

  6. what is the morality of the product I9278, Insulin solution human?

    1 answer

    1. The molarity of a 10 mg/mL solution of human insulin, with a molecular weight of 5807 daltons, is approximately 1.7 μmol/mL or 1.7 mM.

      Helpful?

  7. What kind of solution is the insulin (Product number: I9278) dissolved in?

    1 answer

    1. The insulin (cat# I9278) is dissolved in HEPES solution.

      Helpful?

  8. Are there any contaminants (IgG, IgM example) in I9278?

    1 answer

    1. Unfortunately, this product is not tested for impurities.

      Helpful?

  9. What is the content of the insulin solution/buffer (any perservatives and non insulin components?)

    1 answer

    1. This product is prepared in a HEPES buffer, pH 7.9 - 8.5.

      Helpful?

  10. Can you store this product aliquoted at -20 degrees to extend shelf-life?

    1 answer

    1. For research applications, freezing insulin solution has been a common pratice. However, solutions available on the commercial market for therapeutic use are never frozen, in order to maintain maximum activity. There have been no stability studies perform on the shelf life of frozen insulin solutions. Please see the link below to review a publication that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721649/

      Helpful?

1–10 of 15 Questions  

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