I6160

ITK (352-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): EMT, LYK, MG C126257, MGC126258, PSCTK2
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: buffered aqueous glycerol solution
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: ~53 kDa

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 49-67 nmol/min·mg
• mol wt: ~53 kDa
• UniProt accession no.: Q08881
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... ITK(3702)

Description

General description

ITK (IL2-inducible T-cell kinase) is a member of the Tec family of tyrosine kinases. ITK is expressed in T-cells.[1]

Biochem/physiol Actions

ITK (IL2-inducible T-cell kinase) is important for T-cell activation through the T-cell antigen receptor.[1] Absence of ITK in T cells causes impairment in TCR (T cell receptor)-mediated actin polymerization, cell polarization and signaling events associated with cytoskeletal reorganization.[2]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)