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I1408

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Synonym(s):

Affinity Gel

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5 mL
Please contact Customer Service for Availability
CZK 3,540.00
25 mL
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CZK 11,200.00

About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

CZK 3,540.00

Please contact Customer Service for Availability
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Quality Segment

200

form

(1:1 suspension in a 30% ethanol solution)

shelf life

1 yr

technique(s)

affinity chromatography: suitable

impurities

≤0.08 Metal Contamination (μmol/ml packed gel)

matrix

6% beaded agarose

capacity

10-30 μmol/mL binding capacity (Cu+2)

shipped in

wet ice

storage temp.

2-8°C

Application

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Other Notes

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

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This Item
H8162P9740H0537
IMAC-Select Affinity Gel (1:1 suspension in a 30% ethanol solution)

Sigma-Aldrich

I1408

IMAC-Select Affinity Gel

HIS-Select® Cobalt Affinity Gel (1:1 suspension in a 30% ethanol solution)

Millipore

H8162

HIS-Select® Cobalt Affinity Gel

PHOS-Select™ Iron Affinity Gel

Sigma-Aldrich

P9740

PHOS-Select™ Iron Affinity Gel

HIS-Select® HF Nickel Affinity Gel

Millipore

H0537

HIS-Select® HF Nickel Affinity Gel

matrix

6% beaded agarose

matrix

6% Beaded Agarose

matrix

-

matrix

Highly cross-linked 6% Beaded Agarose

technique(s)

affinity chromatography: suitable

technique(s)

protein purification: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable

form

(1:1 suspension in a 30% ethanol solution)

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

form

suspension

capacity

10-30 μmol/mL binding capacity (Cu+2)

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

120.2 °F - closed cup

flash_point_c

49 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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