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H0537

HIS-Select® HF Nickel Affinity Gel

Synonym(s):

Ni-NTA HF resin

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1 mL
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CZK 2,040.00
CZK 1,734.00
10 mL
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CZK 7,890.00
25 mL
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CZK 14,400.00
100 mL
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CZK 46,100.00
500 mL
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CZK 187,000.00

About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

CZK 1,734.00

List PriceCZK 2,040.00Save 15%
Please contact Customer Service for Availability
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form

suspension

Quality Segment

200

technique(s)

affinity chromatography: suitable

matrix

Highly cross-linked 6% Beaded Agarose

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

storage temp.

2-8°C

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Application

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of HIS TAG® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

  • High selectivity for higher purity
  • Unique non-charged hydrophilic linkage reduced non-specific binding
  • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
  • Highly cross-linked agarose matrix allows for purification under higher pressures
  • Binding under denaturing or non-denaturing conditions
  • One-step purification

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Legal Information

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
P6611H8162P9740
HIS-Select® HF Nickel Affinity Gel

Millipore

H0537

HIS-Select® HF Nickel Affinity Gel

HIS-Select® Nickel Affinity Gel (1:1 suspension in a 20% ethanol solution)

Millipore

P6611

HIS-Select® Nickel Affinity Gel

HIS-Select® Cobalt Affinity Gel (1:1 suspension in a 30% ethanol solution)

Millipore

H8162

HIS-Select® Cobalt Affinity Gel

PHOS-Select™ Iron Affinity Gel

Sigma-Aldrich

P9740

PHOS-Select™ Iron Affinity Gel

matrix

Highly cross-linked 6% Beaded Agarose

matrix

6% Beaded Agarose

matrix

6% Beaded Agarose

matrix

-

technique(s)

affinity chromatography: suitable

technique(s)

protein purification: suitable

technique(s)

protein purification: suitable

technique(s)

-

form

suspension

form

(1:1 suspension in a 20% ethanol solution)

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

target_organs

Eyes,Central nervous system, Respiratory Tract

wgk

WGK 3

flash_point_f

88.0 °F

flash_point_c

31.1 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

10101-97-0 + 67-56-1

CAS No.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCQ5051
SLCL9242
SLCG1131
SLCB1729
SLBR9492V

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Global Trade Item Number

SKUGTIN
H0537-100ML04061833663356
H0537-25ML04061833663387
H0537-500ML04061833663400
H0537-1ML04061833663370
H0537-10ML04061833663363

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