GS59
GST M5-5, Recombinant Human
Synonym(s):
glutathione S-transferase mu 5
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μg | Please contact Customer Service for Availability | CZK 10,500.00 |
About This Item
NACRES:
NA.55
UNSPSC Code:
12352200
Specific activity:
183.9 units/mg protein
Assay:
>95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Concentration:
3.2 mg/mL
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
>95% (SDS-PAGE)
form
frozen liquid
specific activity
183.9 units/mg protein
mol wt
27.4 kDa
concentration
3.2 mg/mL
storage temp.
−70°C
Gene Information
human ... GSTM5(2949)
General description
using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.
Biochem/physiol Actions
Glutathione S-transferase mu 5 (GSTM5) is an enzyme that in humans is encoded by the GSTM5 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
The GSTM5 gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual′s susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.
Preparation Note
The enzyme should be used by the end-user customer within 1 year of receipt.
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information human ... GSTM5(2949) | Gene Information human ... GSTM3(2947) | Gene Information human ... GSTM1(2944) | Gene Information human ... GSTP1(2950) |
| assay >95% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay >95% (SDS-PAGE) |
| specific activity 183.9 units/mg protein | specific activity 5.72 units/mg protein | specific activity 97.96 units/mg protein | specific activity 60.45 units/mg protein |
| biological source human | biological source human | biological source human | biological source human |
| form frozen liquid | form frozen liquid | form frozen liquid | form frozen liquid |
| concentration 3.2 mg/mL | concentration 2.95 mg/mL | concentration 3.1 mg/mL | concentration - |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Christian Delles et al.
Journal of hypertension, 26(7), 1343-1352 (2008-06-14)
Glutathione S-transferases are involved in defences against oxidative stress. We have recently demonstrated reduced expression of glutathione S-transferase mu type 1 (Gstm1) in a rat model of hypertension. Here, we examine the association between GSTM variants and hypertension in human.
Ann M Moyer et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 19(3), 811-821 (2010-03-05)
Lung cancer is commonly treated with platinum compounds. The "glutathione pathway" participates in the metabolism of platinum compounds. We set out to test the hypotheses that single nucleotide polymorphisms (SNPs) or copy number polymorphisms for genes within the glutathione pathway
Y V Patskovsky et al.
Biochemistry, 38(49), 16187-16194 (1999-12-10)
The hGSTM3 subunit, which is preferentially expressed in germ-line cells, has the greatest sequence divergence among the human mu class glutathione S-transferases. To determine a structural basis for the catalytic differences between hGSTM3-3 and other mu class enzymes, chimeric proteins
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GS59-100UG | 04061833655429 |