GLN1

Glutamine and Glutamate Determination Kit

• Synonym(s): L-glutamine enzymatic quantification kit
• NACRES: NA.75
• UNSPSC Code: 12352207

Properties

• usage: kit sufficient for 50 tests
• Quality Level: 200
• packaging: pkg of 1 kit
• storage condition: dry at room temperature
• technique(s): UV/Vis spectroscopy: suitable
• λmax: 340 nm
• application(s): cell analysis; detection
• storage temp.: 2-8°C

Description

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.

Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:

• in minimum essential medium (MEM) medium
• in the supernatant
• in plasma

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD⁺ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3
• hcodes: H302,H311,H314,H317,H330,H334,H350,H410
• pcodes: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1