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Glycerol 3-phosphate Oxidase from Pediococcus sp.

Name: Glycerol 3-phosphate Oxidase from <I>Pediococcus</I> sp.
Brand: SIGMA
Price: 3290 CZK

lyophilized powder, 40-80 units/mg solid, pH 8.1

Synonym(s):

sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase

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Pack Size	SKU	Availability	Price
100 units		Please contact Customer Service for Availability	CZK 3,290.00

About This Item

CAS Number:

9046-28-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-932-0

MDL number:

MFCD00131209

EC Number:

1.1.3.21 (BRENDA, IUBMB)

Specific activity:

40-80 units/mg solid, pH 8.1

Biological source:

bacterial (Pediococcus spp.)

CZK 3,290.00

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Properties

biological source

bacterial (Pediococcus spp.)

Quality Segment

200

form

lyophilized powder

specific activity

40-80 units/mg solid, pH 8.1

mol wt

~76 kDa (gel filtration)

storage temp.

2-8°C

Description

General description

Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.

Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 ^-3M (L-α-Glycerophosphate), 6.8 x 10 ^-3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg⁺⁺, Ag⁺
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40o^C
pH Stability : pH 6.5 - 8.5 (25o^C, 20hr) Thermal stability : below 40oC(pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate

Application

Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.

This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.

Physical form

Lyophilized powder containing stabilizers

Other Notes

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H₂O₂ per min at 37°C, at the appropriate pH.

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This Item	SAE0051	G9888	G3512
Sigma-Aldrich G9637 Glycerol 3-phosphate Oxidase from Pediococcus sp.	Sigma-Aldrich SAE0051 Glycerol 3-phosphate Oxidase from Enterococcus sp.	Sigma-Aldrich G9888 Glycerol 3-phosphate Oxidase from Aerococcus viridans	Sigma-Aldrich G3512 Glycerol Dehydrogenase from Cellulomonas sp.
specific activity 40-80 units/mg solid, pH 8.1	specific activity ≥35 unit/mg solid	specific activity ≥70 units/mg solid	specific activity ≥50 units/mg protein
biological source bacterial (Pediococcus spp.)	biological source -	biological source -	biological source bacterial (Cellulomonas spp.)
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt ~76 kDa (gel filtration)	mol wt -	mol wt -	mol wt ~390 kDa
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200