F5803

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

• Synonym(s): 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B
• Empirical Formula (Hill Notation): C₁₄H₁₆N₂O₂ · 2HCl
• CAS Number: 20325-40-0
• Molecular Weight: 317.21
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24894894
• EC Number: 243-737-5
• Beilstein/REAXYS Number: 3917996
• MDL number: MFCD00012488

Properties

• Quality Segment: 200
• form: crystalline
• pH range: 6.95-7.05
• mp: 268 °C
• solubility: water: 2.5 mg/mL, colorless
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2
• InChI: 1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H
• InChI key: UXTIAFYTYOEQHV-UHFFFAOYSA-N

Description

General description

A preweighed vial containing 50 mg o-Dianisidine.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:

• As substrate for the determination of serum ceruloplasmin from its oxidase activity.[1]
• Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.[2]
• As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).[3]
• Myeloperoxidase activity assay.[4]

Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Disclaimer

Probably carcinogenic.

Safety Information

• pictograms: GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H302,H314,H350
• pcodes: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges